一、基礎(chǔ)信息
貨號：RCP-30-5A
英文名稱：SPHERO™ Rainbow Calibration Particles, 8 Peaks, 3.0-3.4 μm
中文名稱：SPHERO™彩虹校準(zhǔn)微球（8峰，3.0-3.4微米）
品牌：Spherotech
規(guī)格：10?個/mL，5mL/瓶
中國代理商：上海起發(fā)實驗試劑有限公司
Spherotech成立于1992年，總部位于美國伊利諾伊州，是微球產(chǎn)品制造商，專注于為生物醫(yī)學(xué)、診斷和藥物開發(fā)領(lǐng)域提供均一化微球解決方案。其產(chǎn)品以高精度、高穩(wěn)定性和廣泛適用性著稱，被全球科研機(jī)構(gòu)、生物技術(shù)公司及醫(yī)療機(jī)構(gòu)廣泛采用。
二、產(chǎn)品原理
SPHERO™彩虹校準(zhǔn)微球通過多色熒光標(biāo)記技術(shù)實現(xiàn)對儀器的全面校準(zhǔn)。每個微球內(nèi)部均勻嵌入多種熒光染料（如FITC、PE、PE-Cy5、APC等），形成8個熒光強(qiáng)度梯度的峰。這些熒光染料的激發(fā)波長覆蓋365-650nm，可適配主流流式細(xì)胞儀的激光系統(tǒng)（如488nm、561nm、633nm等）。
其核心原理包括：
1. 多通道同步校準(zhǔn)：
單一微球可同時校準(zhǔn)流式細(xì)胞儀的前向散射（FSC）、側(cè)向散射（SSC）及多個熒光通道（如FL1-FL5），通過檢測8個熒光峰的位置和強(qiáng)度，驗證儀器的靈敏度、線性度和分辨率。
2. 熒光穩(wěn)定性設(shè)計：
染料通過物理包埋而非表面偶聯(lián)的方式固定在微球內(nèi)部，避免了外界環(huán)境（如pH、溫度、酶解）對熒光信號的干擾，確保長期儲存和重復(fù)使用時的信號一致性。
3. 對數(shù)放大器線性驗證：
微球的熒光強(qiáng)度呈對數(shù)分布，可通過配套的Excel模板（如SPHERO™ PMT Quality Control Template）生成校準(zhǔn)曲線，定量評估儀器對數(shù)放大器的線性誤差，確保檢測結(jié)果的準(zhǔn)確性。
三、解決實驗中的關(guān)鍵問題
1. 儀器標(biāo)準(zhǔn)化與質(zhì)量控制：
- 流式細(xì)胞儀的光路校準(zhǔn)：通過FSC和SSC信號調(diào)整液流穩(wěn)定性和激光對齊，確保細(xì)胞大小和顆粒度檢測的準(zhǔn)確性。
- 熒光通道靈敏度驗證：檢測微球的熒光強(qiáng)度閾值，確認(rèn)儀器對弱信號的檢測能力，避免因PMT電壓設(shè)置不當(dāng)導(dǎo)致的假陰性結(jié)果。
- 線性度評估：驗證對數(shù)放大器在寬動態(tài)范圍內(nèi)的線性響應(yīng)，確保高表達(dá)和低表達(dá)樣本的信號均能準(zhǔn)確區(qū)分。
2. 實驗數(shù)據(jù)的可比性與重復(fù)性：
- 多實驗室間數(shù)據(jù)標(biāo)準(zhǔn)化：通過統(tǒng)一使用SPHERO™彩虹校準(zhǔn)微球，可消除不同儀器和實驗條件對結(jié)果的影響，實現(xiàn)跨平臺數(shù)據(jù)的可比性。
- 長期實驗質(zhì)量跟蹤：定期校準(zhǔn)可監(jiān)測儀器性能變化，及時發(fā)現(xiàn)光路偏移、激光衰減等問題，避免實驗結(jié)果的系統(tǒng)性偏差。
3. 復(fù)雜實驗設(shè)計的優(yōu)化：
- 熒光補(bǔ)償設(shè)置：通過微球的多色熒光特性，快速建立補(bǔ)償矩陣，減少因熒光光譜重疊導(dǎo)致的信號干擾。
- 多色實驗通道匹配：根據(jù)微球的熒光發(fā)射譜，合理選擇熒光染料組合，提高實驗檢測維度。
四、核心優(yōu)勢
1. 多用途兼容性：
- 適用于主流流式細(xì)胞儀品牌（如BD、Beckman Coulter、Cytek等），支持從科研級到臨床級儀器的校準(zhǔn)需求。
- 除流式細(xì)胞儀外，還可用于熒光顯微鏡、共聚焦顯微鏡等設(shè)備的光路校準(zhǔn)和熒光強(qiáng)度標(biāo)準(zhǔn)化。
2. 高穩(wěn)定性與可靠性：
- 熒光信號在2-8℃儲存條件下可穩(wěn)定保存12個月以上，且耐受反復(fù)凍融（至少10次），降低實驗成本。
- 微球粒徑均一性高（CV<2%），確保檢測結(jié)果的重復(fù)性和統(tǒng)計學(xué)意義。
3. 操作簡便與經(jīng)濟(jì)性：
- 即用型設(shè)計，無需復(fù)雜預(yù)處理，直接取1-2滴微球懸液稀釋后即可上機(jī)檢測。
- 每瓶可進(jìn)行約50-100次校準(zhǔn)（根據(jù)儀器進(jìn)樣量調(diào)整），單次實驗成本顯著低于同類產(chǎn)品。
4. 技術(shù)支持與資源豐富：
- 提供詳細(xì)的技術(shù)文檔（如STN-8、STN-14）和操作視頻，指導(dǎo)用戶完成校準(zhǔn)流程及數(shù)據(jù)分析。
- 配套Excel模板可自動生成校準(zhǔn)報告，簡化數(shù)據(jù)處理步驟。
五、客戶群體與典型應(yīng)用
1. 科研領(lǐng)域：
- 免疫分析：T細(xì)胞亞群分型、細(xì)胞因子檢測、抗體效價評估等實驗中，確保流式細(xì)胞儀的靈敏度和線性度。
- 細(xì)胞生物學(xué)：干細(xì)胞分化研究、細(xì)胞凋亡檢測、細(xì)胞周期分析等，保證實驗數(shù)據(jù)的準(zhǔn)確性和可重復(fù)性。
- 癌癥研究：循環(huán)腫瘤細(xì)胞（CTC）計數(shù)、腫瘤微環(huán)境分析等，對稀有細(xì)胞的檢測精度要求高。
2. 臨床診斷與IVD：
- 血液學(xué)檢測：白血病免疫分型、血小板功能分析等，需嚴(yán)格遵循臨床實驗室標(biāo)準(zhǔn)化流程。
- 感染性疾病診斷：HIV病毒載量監(jiān)測、結(jié)核桿菌抗原檢測等，確保檢測結(jié)果的一致性和合規(guī)性。
3. 工業(yè)質(zhì)檢與生物技術(shù)：
- 生物制藥：重組蛋白表達(dá)分析、病毒載體滴度測定等，對儀器的穩(wěn)定性和準(zhǔn)確性要求很高。
- 納米材料表征：納米顆粒粒徑分布檢測、熒光標(biāo)記效率評估等，利用微球的熒光特性進(jìn)行定量分析。
六、訂貨信息
1. 產(chǎn)品規(guī)格：
- 貨號RCP-30-5A：8峰，3.0-3.4μm，10?個/mL，5mL/瓶
- 其他可選規(guī)格：
- RCP-30-5：6峰，3.0-3.4μm，5mL
- RCP-20-5：4峰，1.8-2.2μm，5mL
- 超彩虹系列（Ultra Rainbow）：適用于紫外和遠(yuǎn)紅外通道校準(zhǔn)
2. 儲存條件：
- 2-8℃避光保存，避免冷凍。未開封狀態(tài)下有效期為12個月，開封后建議6個月內(nèi)使用完畢。
3. 訂貨渠道：
- 中國代理：上海起發(fā)實驗試劑有限公司
4. 注意事項：
- 使用前需充分混勻微球懸液，避免因沉淀導(dǎo)致的濃度不均。
- 校準(zhǔn)過程中需使用過濾后的去離子水或鞘液，防止雜質(zhì)干擾檢測結(jié)果。
- 如發(fā)現(xiàn)微球懸液出現(xiàn)渾濁或沉淀，應(yīng)停止使用并聯(lián)系供應(yīng)商更換。
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更多產(chǎn)品信息，請聯(lián)系中國區(qū)域代理商：上海起發(fā)實驗試劑有限公司
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（注：本文內(nèi)容基于品牌公開資料及行業(yè)常規(guī)信息整理，具體以品牌信息文檔為準(zhǔn)。）
??Spherotech熱賣產(chǎn)品
貨號 | 品名 | 規(guī)格 | 品牌 |
RCP-30-5A | Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL | 5mL | Spherotech |
URFP-30-2 | UltraRainbow Fluorescent Particles 1E7/mL 3.0-3.4 um | 2ml | Spherotech |
NVP-60-5 | Neutravidin Polystyrene Particles 0.5％ w/v 6.0-8.0 um 5 mL | 5mL | Spherotech |
MPFc-60-5 | Gt anti-Ms IgG (Fc) Polystyrene Particles, 0.5％w/v, 6.0-8.0µm, 5mL | 5ml | Spherotech |
URCP-38-2K | UltraRainbow Calibration Particle Kit, 3.8 +/- 0.2µm,2E6/mL, 1x3mL (blank) & 1E7/mL, 1x2mL (fluorescent) | 1x3mL | Spherotech |
SVP5-60-5 | Streptavidin Polystyrene Particles, 5%w/v, 6.0-8.0µm, 5mL | 5mL | Spherotech |
SVP-60-5 | Streptavidin Polystyrene Particles, 0.5％w/v, 6.0-8.0μm | 5mL | Spherotech |
C62930 | Calibration Beads for MoFlo Astrios, 1E7/mL, 3.0-3.4µm, 2 mL | 2mL | Spherotech |
HUP-60-5 | Gt anti-Hu IgG(H&L) Polystyrene Particles, 0.5％w/v, 6.0-7.9μm, 5mL | 5mL | Spherotech |
RCP-30-5 | Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-30-5) | 5mL | Spherotech |
RPFc-60-5 | Goat anti-Rabbit IgG (Fc) Coated Polystyrene Particles 0.5% w，6.0-8.0µm | 5 mL | Spherotech |
DIGP-20-2 | Anti-Digoxigenin Coated Polystyrene Particles, 2.0- 2.4µm, 0.1％w/v, 2mL | 2mL | Spherotech |
ACBP-70-10 | AccuCount Blank, 10^6/mL, 7.0-7.9μm, 10mL | 10mL | Spherotech |
NVM-20-5 | NeutrAvidin Coated Magnetic Particles | 5mL | Spherotech |
NFPPS-52-4K | NANO FLUORESCENT SIZE STANDARD, FLOW CYTOMETRY GRADE, YELLOW, NFPPS系列 | 4x5mL | Spherotech |
URQP-38-6K | Ultra Rainbow Quantitative Particle Kit | 6x2mL | Spherotech |
urfp-100-2 | ULTRARAINBOW FLUORESCENT PARTICLES, URFP系列超級彩虹熒光微球 | 2ml | Spherotech |
SVP-50-5 | Streptavidin Polystyrene Particles, 0.5%w/v, 5.0-5.9µm, 5mL | 5mL | Spherotech |
SVP-40-5 | Streptavidin Coated Polystyrene Particles 0.5％ w/v 4.0-4.9 μm | 5mL | Spherotech |
SVP-10-5 | Streptavidin Polystyrene Particles, 1%w/v, 1.0-1.4um, 5mL | 5mL | Spherotech |
SVP-08-10 | Streptavidin Polystyrene Particles 1％ w/v 0.7-0.9um | 10mL | Spherotech |
SVM-025-5H | Streptavidin Magnetic Particles, High Iron, 0.5％ w/v, 0.2-0.39 um, 5 mL | 5mL | Spherotech |
ACFP-50-5 | AccuCount Fluorescent Particles, 10^6/mL, 5.0-5.9µm, 5mL | 5mL | Spherotech |
MPFc-30-5 | Gt anti-Ms IgG (Fc) Polystyrene Particles, 0.5％w/v, 3.0-3.9µm, 5mL | 5mL | Spherotech |
PPS-6K | Polystyrene Particle Size Standard Kit, Flow Cytometry Grade, 2.5E6/mL, 6x5mL, 2.0-2.4µm, 3.0-3.4µm, 5.0-5.9µm, 7.0-7.9µm, 8.0-12.9µm, & 13.0- 17.9µm | 6x5mL | Spherotech |
CMIGP-70-3K | COMPtrol Gt anti-Ms Ig(H&L) Particles Kit, 7.0-7.9µm., 3X5mL | 3X5mL | Spherotech |
CMX-200-10 | Carboxyl Magnetic Particles, Crosslinkled, 1%w/v, 18.0-22.9µm, 10mL | 10mL | Spherotech |
FP-00556-2 | Fluorescent Particles, Nile Red, 1%w/v, 0.04-0.06µm, 2mL | 2mL | Spherotech |
CMX-300-1000 | Carboxyl Magnetic Particles, Crosslinked,1％ w/v, 25-37 um, 1000 mL | 1000mL | Spherotech |
SVP-15-5 | Streptavidin Polystyrene Particles 1％ w/v 1.5-1.9um 5 mL | 5mL | Spherotech |
CP-10-10 | Carboxyl Polystyrene Particles, 5％w/v, 1.0-1.4µm, 10mL | 10mL | Spherotech |
DDCP-70-2 | Drop Delay Calibration Particles | 2mL | Spherotech |
DIGP-40-2 | Anti-Digoxigenin Coated Polystyrene Particles, 4.0- 4.9um, 0.1%w/v, 2mL | 2mL | Spherotech |
PGP-08-5 | Protein G Polystyrene Particles, 1%w/v, 0.7-0.9µm, 5mL | 5mL | Spherotech |
PAP-60-5 | Protein A Polystyrene Particles, 0.5%w/v, 6.0-8.0um, 5mL | 5mL | Spherotech |
PAP-40-5 | Protein A Polystyrene Particles, 0.5%w/v, 4.0-4.9um, 5mL | 5mL | Spherotech |
PAP-08-5 | Protein A Polystyrene Particles, 1%w/v, 0.7-0.9µm, 5mL | 5mL | Spherotech |
PGP-30-5 | Protein G Polystyrene Particles, 0.5％w/v, 3.0-3.4µm, 5mL | 5mL | Spherotech |
PM-40-10 | Polystyrene Magnetic Particles, 2.5%w/v, 4.0-4.5µm, 10mL | 10mL | Spherotech |
PP-08-10 | polystyrene particles，5％w/v，0.7-0.9um | 10ml | Spherotech |
PP-100-10 | Polystyrene Particles, 2.5%w/v, 8.0-12.9um, 10mL | 10mL | Spherotech |
PP-20-10 | Polystyrene Particles, 5%w/v, 2.0-2.4µm, 10mL | 10mL | Spherotech |
PP-30-10 | Polystyrene Particles, 5%w/v, 3.0-3.4µm, 10mL | 10mL | Spherotech |
PP10-10-10 | Polystyrene Particles, 10％w/v, 1.0-1.4µm, 10mL | 10mL | Spherotech |
MP-60-5 | Gt anti-Ms IgG (H&L) Polystyrene Particles, 0.5％w/v, 6.0-8.0µm, 5mL | 5mL | Spherotech |
ACBP-20-10 | AccuCount Blank Particles | 10mL | Spherotech |
MMSFc-40-10 | Gt anti-Ms IgG (Fc) Magnetic Particles, Smooth, 1％w/v, 4.0-5.0um, 10mL | 10mL | Spherotech |
PPX-600-10 | Polystyrene Particles, Crosslinked, Research Grade, 5%w/v, 53.0-69.0µm, 10mL | 10mL | Spherotech |
MM-40-10 | Gt anti-Ms IgG (H&L) Magnetic Particles, 1％w/v, 4.0-4.9μm, 10mL | 10mL | Spherotech |
HUP-100-5 | Gt anti-Hu IgG(H&L) Polystyrene Particles, 0.5%w/v, 10.0-14.0µm, 5mL | 5mL | Spherotech |
HMX-10-10 | Gt anti-Hu IgG (H&L) Magnetic Particles, Crosslinked, 0.5%w/v, 1.0-2.0um, 10mL | 10mL | Spherotech |
FSVM-8058-2 | Streptavidin Fluorescent Magnetic Particles, Pink, 0.1％w/v, 8.0-9.9μm, 2mL | 2mL | Spherotech |
FSVM-2058-2 | Streptavidin Fluorescent Magnetic Particles, Pink, 0.1％w/v, 2.0-2.9μm, 2mL | 2mL | Spherotech |
FSVM-02556-2 | Streptavidin Fluorescent Magnetic Particles, Nile Red, 0.1％w/v, 0.2-0.39μm, 2mL | 2mL | Spherotech |
FPS-15057 | Fluorescent Particle Slide, Rainbow, 13.0-17.9µm | 13.0-17.9um | Spherotech |
FPS-10057 | Fluorescent Particle Slide, Rainbow, 10.0-14.0µm | 10.0-14.0um | Spherotech |
RCP-30-5A-8 | Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL | 5mL | Spherotech |
RFP-30-5A | Rainbow Fluorescent Particles, Mid-Range, 1E7/mL, 3.0-3.4µm, 5mL | 5mL | Spherotech |
FP-0256-2 | Fluorescent Particles, Nile Red, 1%w/v, 0.1-0.3µm, 2mL | 2mL | Spherotech |
RPFc-08-20 | Gt anti-Rb IgG (Fc) Polystyrene Particles, 0.25%w/v, 0.7-0.9µm, 20mL | 20mL | Spherotech |
RTPFC-60-5 | Goat anti-Rat IgG (Fc) Coated Polystyrene Particles, Cross-adsorbed, 0.5%w/v, 6.0-8.0um, 5mL | 5mL | Spherotech |
FH-3056-2 | Fluorescent Particles, Nile red, High Intensity, 1%w/v, 2.5-4.5µm, 2mL | 2mL | Spherotech |
SVFM-20-5 | Streptavidin Ferromagnetic Particles, 1%w/v, 2.0-2.9um, 5mL | 5mL | Spherotech |
SVFP-0556-5 | Streptavidin Fluorescent Particles, Nile Red, 0.1％w/v, 0.4-0.6μm 5mL | 5mL | Spherotech |
SVFP-6056-5 | Streptavidin Fluorescent Particles, Nile Red, 0.1%w/v, 5.0-7.9µm, 5mL | 5mL | Spherotech |
SVFP-6062-5 | Streptavidin Fluorescent Particles, Purple, 0.1%w/v, 5.0-7.9µm, 5mL | 5mL | Spherotech |
DIGP-08-2 | Anti-Digoxigenin Coated Polystyrene Particles, 0.7- 0.9um, 0.1%w/v, 2mL | 2mL | Spherotech |
CMX-300-1 | Carboxyl Magnetic Particles, Crosslinked, 1％ w/v，25-37um | 2x 1mL Samples | Spherotech |
URCP-100-2 | UltraRainbow Calibration Particles, 6 peaks, 8.0-12.9um, 1E7/mL, 2mL | 2mL | Spherotech |
TM-40-10 | Biotin Magnetic Particles, 1％w/v, 4.0-4.5µm, 10mL | 10mL | Spherotech |
TFP-0556-5 | Biotin Fluorescent Particles, Nile Red, 0.1％ w/v, 0.4-0.6 um, 5 mL | 5mL | Spherotech |
URCP-100-2-1 | Ultra Rainbow Calibration Particle, Peak 1, 8.0 - 12.9 um, 1E7/mL | 2mL | Spherotech |
CM-025-10H | Carboxyl Magnetic Particles, High Iron, 1.0％ w/v, 0.1-0.39 um, 10 mL | 10mL | Spherotech |
CFM-80-5 | Carboxyl Ferromagnetic Particles, 1％w/v, 8.0-8.9µm, 5mL | 5mL | Spherotech |
CM-05-10H | Carboxyl Magnetic Particles, High Iron, 1.0％w/v, 0.4-0.69µm, 10mL | 10mL | Spherotech |
URCP-100-2-6 | SPHERO Ultra Rainbow Calibratior Particles,Peak 6,10.4 um, 2 mL | 2mL | Spherotech |
URCP-100-2-8 | SPHEROm Ultra Rainbow CalibrationParticles，Peak 8,10.6 um, | 2mL | Spherotech |
CM-200-10 | Carboxyl Magnetic Particles, 1％w/v, 18.0-22.9µm, 10mL | 10mL | Spherotech |
CFM-20-10 | Carboxyl Ferromagnetic Particles, 1％w/v, 2.0-2.9µm, 10mL | 10mL | Spherotech |
CM-60-10 | Carboxyl Magnetic Particles, 2.5％w/v, 6.0-8.0µm, 10mL | 10mL | Spherotech |
URFP-02-2 | Ultra Rainbow Fluorescent Particles, ~1E10/mL, 0.1-0.3µm, 2mL | 2mL | Spherotech |
AUP5-002-20 | Citrate Gold Particles, 5.0nM, 15-20nm, 20mL | 20mL | Spherotech |
CMPAK-4068-6K | Carboxyl Magnetic Blue Particle Array Kit, 6 Peaks, 1E7/mL, 4.0-4.9 um, 6x1 mL | 6x1mL | Spherotech |
CMPAK-5068-6K | Carboxyl Magnetic Blue Particle Array Kit, 6 peaks 107/mL 4.9-5.9 μm | 6x1mL | Spherotech |
CMX-300-10 | Carboxyl Magnetic Particles Crosslinked 1％ w/v 25-37 um 10 mL | 10mL | Spherotech |
VM-40-10 | Avidin Magnetic Particles, 1％w/v, 4.0-4.5µm, 10mL | 10mL | Spherotech |
CMX-40-10 | Carboxyl Magnetic Particles, Crosslinked, 2.5%w/v, 3.0-6.0µm, 10mL | 10mL | Spherotech |
ACURFP-38-5 | ACCUCOUNT ULTRARAINBOW FLUORESCENT PARTICLES | 5ml | Spherotech |
CP-15-10 | Carboxyl Polystyrene Particles, 5%w/v, 1.5-1.9um, 10mL | 10mL | Spherotech |
CP-20-10 | Carboxyl Polystyrene Particles, 5%w/v, 2.0-2.4um, 10mL | 10mL | Spherotech |
SVMS-30-10 | Streptavidin Magnetic Particles, Smooth, 1%w/v, 3.0-3.9µm, 10mL | 10mL | Spherotech |
SVP-100-4 | Streptavidin Polystyrene Particles, 0.5％ w/v, 10.0-14.0 um, 4 mL | 4mL | Spherotech |
CP-25-10 | Carboxyl Polystyrene Particles, 5%w/v, 2.5-2.9um, 10mL | 10mL | Spherotech |
SVMX-300-4 | Streptavidin Coated Magnetic Particles, Crosslinked 0.5％ w/v 25.0-37.0 µm | 4mL | Spherotech |
AM-05-10H | Amino Superparamagnetic Particles, High iron 1.0％w/v, 0.4-0.69um, 10mL | 10mL | Spherotech |
VP-10-10 | Avidin Polystyrene Particles, 5.0%w/v, 1.0-1.9µm, 10mL | 10mL | Spherotech |
VP-30-5 | Avidin Polystyrene Particles, 0.5%w/v, 3.0-3.9µm, 5mL | 5mL | Spherotech |
PGP-400-5 | Protein G Polystyrene Particles, 1％w/v, 38-52µm, 5mL | 5mL | Spherotech |
VP-60-5 | Avidin Polystyrene Particles, 0.5%w/v, 6.0-8.0µm, 5mL | 5mL | Spherotech |
